When freezing the tissues and organs for transplantation, it is important to prevent the danger formation of ice crystals. The new technology created by US scientists allows to solve this problem. They were the first to successfully applied the cooling method to preserve and revitalize artificially created heart fabrics. This means that in the future, donor bodies can stay more suitable for transplant.
The technology proposed by experts from the University of California in Berkeley is called isochoric cooling and was invented 16 years ago. It avoids the formation of ice crystals in the process of preserving biological material, and also begins to penetrate the food industry, where it promises a huge energy savings.
While traditional isobaric cooling freezes the material under the action of constant pressure in the air, isochoric requires immersion in the liquid. The material is then sealed in a solid container without air and excess space for the formation of ice crystals. In the past, scientists have already managed to cool the samples to -22 ° C and prevent freezing of 40% of the material,
Now the researchers have developed a heart system on a chip, growing the tissue of the heart from adult stem cells. These fabrics are reduced in the same rhythm as the human heart, and microfluidal channels serve to supply nutrients and drugs. All this they immersed in an isochoric chamber and cooled to -3 ° C. Then the samples were dismissed after 24, 48 and 72 hours, heating them to + 37 ° C.
Spontaneous heartbeat has been preserved in 65-80% of samples, without a significant difference in the cooling duration. The material analysis showed that the structural integrity of the tissue did not change. Also, the samples have retained susceptibility to isoproterenol, which increases the frequency of heart abbreviations.
Successful defrosting of heart tissues after three cooling days — promising proof of technology efficiency. It significantly increases the timing of the possible transfers of the bodies, which are now measured in hours.
Breakthrough in the cultivation of donor organs
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